Paschalis EP¹, Gamsjaeger S², Hassler N², Fahrleitner-Pammer A³, Dobnig H⁴, Stepan JJ⁵, Pavo I⁶, Eriksen EF⁶, Klaushofer K².

Author information

• ¹Ludwig Boltzmann Institute of Osteology, Hanusch Hospital of WGKK, AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Heinrich Collin Str. 30, A-1140, Vienna, Austria. Electronic address: lpaschalis@gmx.net.
• ²Ludwig Boltzmann Institute of Osteology, Hanusch Hospital of WGKK, AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Heinrich Collin Str. 30, A-1140, Vienna, Austria.
• ³Dept. of Internal Medicine, Medical University Graz, Austria.
• ⁴Thyroid, Endocrinology, and Osteoporosis Institute, Graz, Austria.
• ⁵Institute of Rheumatology, Faculty of Medicine 1, Charles University, Prague, Czech Republic.
• ⁶Endocrinology Dept., Oslo University Hospital, Norway.

Abstract

Prospective, controlled clinical trials in postmenopausal osteoporosis typically compare effects of an active drug with placebo in addition to vitamin D and calcium supplementation in both treatment arms. While clinical benefits are documented, the effect of this supplementation in the placebo arm and in clinical practice on bone material composition properties is unknown. The purpose of the present study was to evaluate these bone quality indices (specifically mineral/matrix, nanoporosity, glycosaminoglycan content, mineral maturity/crystallinity, and pyridinoline content) in patients that either received long-term vitamin D (400-1200IU) and calcium (1.0-1.5g) supplementation, or did not. We have analyzed by Raman microspectroscopy the bone forming trabecular surfaces of iliac crest in pre-treatment samples of a teriparatide study and the endpoint biopsies of the control arm obtained from the HORIZON trial. In general, the mineral/matrix ratio and the glycosaminoglycan (GAG) content was higher while nanoporosity, (a surrogate for tissue water content), the mineral maturity/crystallinity (MMC) and the pyridinoline (Pyd) content was lower in patients without long-term supplementation. Moreover, all indices were significantly dependent on tissue age. In conclusion, vitamin D and calcium supplementation is associated with altered mineral and organic matrix properties.

Copyright © 2016. Published by Elsevier Inc.

KEYWORDS:

Bone quality; Calcium; Postmenopausal osteoporosis; Raman spectroscopy; Vitamin D

PMID: 27826025   DOI: 10.1016/j.bone.2016.11.002

資料來源：PubMed
連結網址：https://www.ncbi.nlm.nih.gov/pubmed/27826025